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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 °C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent Cream/Lotion Quantity:2-Pack Can You Take Too Much Vitamin B12? Overdose and Side Effects | Drip Gym https://ars.els-cdn.com/content/image/1-s2.0-S0269749125006244-ga1.jpg

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