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A structural explanation for these findings was provided from our previous cryo-EM structures of CT(Pro32) and rAmy(Tyr37)-bound AMYRs structures where the bulky phenolic side chain of Y37 rAmy forms perpendicular π stacking interactions with W79 ECD 15 , and additional interactions with the respective RAMP protomer that are likely important for selectivity of rAmy for AMYRs over CTR
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J Pharmacol Exp Ther 2006;319:1485–91 [DOI] [PubMed] [Google Scholar] 17.Zamek-Gliszczynski MJ, Hoffmaster KA, Tian X, Zhao R, Polli JW, Humphreys JE, Webster LO, Bridges AS, Kalvass JC, Brouwer KL.Multiple mechanisms are involved in the biliary excretion of acetaminophen sulfate in the rat: role of Mrp2 and Bcrp1